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KMID : 1147720130060030142
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Journal of Acupuncture and Meridian Studies
2013 Volume.6 No. 3 p.142 ~ p.148
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In vitro Antioxidant Activities of Methanol and Aqueous Extract of Annona squamosa (L.) Fruit Pulp
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Nandhakumar Elumalai
Indumathi Parameswaran
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Abstract
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The present study evaluated the antioxidant activity of the fruit of Annona squamosa by means of in vitro studies involving two different solvent extracts: methanol and aqueous. The antioxidant properties of the extract were determined by scavenging 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), lipid peroxidation (LPO), nitric oxide (NO), superoxide anion (O2?), hydroxyl radical (OH?), reducing power and total antioxidant. The results showed that, compared to aqueous extract, a methanolic fruit extract of A squamosa has a higher percentage of inhibition of DPPH radical scavenging activity (97.99%), LPO (94.15%), NO scavenging activity (70.96%), O2? scavenging activity and OH? scavenging activity (78.68% and 85.25%, respectively), total antioxidant activity (206 μg α-tocopherol/g) and reducing power (56.0 μg of ascorbic acid/g). The results obtained in the in vitro models clearly suggest that methanol extract has higher antioxidant activity than the aqueous extract due to a higher presence of phenolic and flavonodal constituents in the methanol extract.
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KEYWORD
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Annona squamosa, antioxidant, ascorbic acid, DPPH, lipid peroxidation, nitric oxide
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